蜂巢矩陣葉綠素熒光成像系統(tǒng)
HEXAGON-IMAGING-PAM
葉綠素熒光成像系統(tǒng)的“六邊形戰(zhàn)士”
精度高，面積大，功能全，應用廣，文獻多，數(shù)據(jù)可視化！
HEXAGON-IMAGING-PAM是德國WALZ公司新推出的大型蜂巢矩陣葉綠素熒光成像系統(tǒng)。它憑借高精度的脈沖振幅調(diào)制（PAM）技術(shù)，可以對20×24cm的區(qū)域進行成像。分辨率高達1.2 MP（1000 x 1200 px, 2x2 binning技術(shù)，實際是2000×2400），像素尺寸3.45 x 3.45 μm。
超高分辨率的基礎(chǔ)是成像區(qū)域光場的均勻性，在設計過程中，光源陣列中LED的位置是經(jīng)過精心布局的，以保證測量區(qū)域內(nèi)無陰影，所有成像區(qū)域內(nèi)的樣品均勻照光，樣品間的差異可以盡收眼底。大功率LED面板的冷卻效果非常好，可以大限度的延長LED的使用壽命。
增加遠紅光（FR）LED 面板，可用于測量所研究樣品的Fo'值。
HEXAGON-IMAGING-PAM采用蜂巢矩陣式LED面板拼接技術(shù)，單個六邊形蜂巢矩陣單元之間LED的不平橫可以獨立補償，初衷是為實現(xiàn)樣品區(qū)域的理想照明提供更優(yōu)的選擇。
盡管成像區(qū)域很大，但是它依然足夠靈活，可以測量各種類型的樣品，如盆栽植物，穴盤中培養(yǎng)的植物，培養(yǎng)皿上的植物或多孔板中的藻類懸浮液。
滑動門設計，集成安全關(guān)閉功能，開門狀態(tài)下，飽和脈沖的強度會被抑制以保護操作人員的眼睛。
主要功能
l 原位測量：活體植物葉綠素熒光成像，直觀顯示樣品光合作用光能利用差異，可導出彩色圖像。
l 成像功能：對Ft、Fo、Fm、Fv/Fm、F、Fm’、Y(II)、Y(NO)、Y(NPQ)、NPQ、qN、qP、qL、PS/50=ETR、Inh等參數(shù)進行成像分析。測定調(diào)節(jié)性能量耗散Y(NPQ)，反映植物光保護能力，測定非調(diào)節(jié)性能量耗散Y(NO)，反映植物光損傷程度。
l 程序測量功能：可自動程序測量熒光誘導曲線、快速光曲線和暗弛豫，也可手動測量；在測量過程中能自動分析所有熒光參數(shù)的變化趨勢；可以預編程進行自定義實驗流程，如模擬波動光。
l AOI功能：可在測量前或測量后任意選擇感興趣的區(qū)域（AOI），程序?qū)⒆詣訉x擇的AOI的數(shù)據(jù)進行變化趨勢分析，并在報告文件中顯示相關(guān)AOI的數(shù)據(jù)。所有報告文件中顯示的數(shù)據(jù)都可導出到EXCEL文件中。
l 成像異質(zhì)性分析功能：對任意參數(shù)任意時間的成像，可在圖像上任意選取兩點，軟件自動對兩點間的數(shù)據(jù)進行橫向異質(zhì)性分析，并可導出到EXCEL文件中。
l 成像數(shù)據(jù)范圍分析功能：對任意參數(shù)任意時間的成像，可分析任意兩個熒光數(shù)值之間有多少個像素點，多少面積（cm2）。
l 突變株篩選功能：可跟據(jù)成像結(jié)果快速篩選光合、產(chǎn)氫/油、抗逆（抗鹽、抗旱、抗病等）等突變株。
l 微藻毒理研究功能：可同時測量4塊96孔板，即384個微藻樣品（對照和處理組）的光合活性，軟件自動給出處理組樣品相對于對照組的光合抑制百分比。
應用領(lǐng)域
l 光合作用研究：可以在完全相同的條件下同時對大量樣品進行成像
l 植物病理學：病斑部位（包括肉眼不可見時）成像以及病斑擴散的時空動力學
l 植物脅迫生理學：肉眼不可見生物/非生物脅迫損傷的早期檢測
l 遺傳育種：出苗后大規(guī)?？焖俸Y選高光合/抗旱/抗熱/抗凍/抗病等植株
l 突變株篩選：快速篩選模式植物的光合突變株、抗逆突變株、產(chǎn)氫微藻突變株等
l 微藻毒理學：不同毒物濃度多個重復的樣品一次測完，軟件自動計算抑制比率
l 其它多種擴展研究
成像參數(shù)
Fo, Fm, F, Ft, Fm', Fv/Fm, Y(II), qL, qP, qN, NPQ, Y(NPQ), Y(NO), PS/50=ETR,Inh.等
產(chǎn)地：德國WALZ
參考文獻
數(shù)據(jù)來源：光合作用文獻Endnote數(shù)據(jù)庫，原始數(shù)據(jù)來源：Google Scholar。
注：HEXAGON-IMAGING-PAM為新產(chǎn)品，暫無文獻發(fā)表，研究成果可參考M-IMAGING-PAM發(fā)表文章。
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